Fluorescence Spectroscopy in Camptothecins Studies

Fluorescence Spectroscopy In Vivo

Comparative studies on the interaction of cefixime with bovine serum albumin by fluorescence quenching spectroscopy and synchronous fluorescence spectroscopy.

Under simulated physiological conditions, the reaction mechanism between cefixime and bovine serum albumin at different temperatures (293, 303 and 310 K) was investigated using a fluorescence quenching method and synchronous fluorescence method, respectively. The results indicated that the fluorescence intensity and synchronous fluorescence intensity of bovine serum albumin decreased regularly ...

Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy.

This article focuses on methods based on fluctuation correlation spectroscopy to determine the formation of protein complexes in living cells. We present the principles of the fluctuation method applied to cells. We discuss the novelty and the promises of this approach. The emphasis is in the discussion of the underlying statistical assumptions of the image correlation spectroscopy analysis rat...

In vitro study of drug-protein interaction using electronic absorption, fluorescence, and circular dichroism spectroscopy

In the near future, design of a new generation of drugs targeting proteins will be required. Considering the complex bond between the drug and protein, the structure and stability of the target protein should be considered. So far, a series of in vitro investigations have been conducted with the aim of predicting drug-biological medium interactions. In these studies, use of spectroscopic method...

Concepts in Fluorescence Correlation Spectroscopy

Fluorescence Correlation Spectroscopy (FCS) and related techniques are becoming widely used to measure molecular dynamics in a broad variety of specimens. These techniques provide important quantitative information on dynamics -such as diffusion coefficients, local concentration, binding reactions under equilibrium conditionswith high spatial and temporal resolutions. Also, they are minimal inv...